碳氮源对红曲霉S产色素的影响

Effects of Carbon and Nitrogen Sources on Monascus purpureus S in its Secreting Pigment

通过液态培养,研究不同种类不同浓度的碳氮源对红曲霉S产色素的影响。结果表明:在本试验条件下,碳源和氮源种类对色价及色调的影响差异均达到极显著水平(1%);碳源的添加浓度为4%～5%时最适宜红曲霉S产色素,而氮源的添加浓度为2.00%～2.40%时最适宜红曲霉S产色素。以甘露醇为单一碳源产生的色素其色价最高,为119.6u.g-1,其次是大米粉,为113.9u.g-1,两者色调均偏红;培养基中添加葡萄糖与蔗糖的产品色调偏黄,但总色价偏低;以硫酸铵为氮源的产品色调偏黄,色价较高;以硝酸铵为氮源的产品肉眼观察属橙红色,色价也在100.0u.g-1以上;以味精和蛋白胨为氮源的产品色调偏红,色价在80.0u.g-1以上;在氮源试验中,根据试验数据,作了如下推测:无机氮源中,NH4+促进红曲霉S产黄色素,NO3-促进红曲霉S产红色素。

Different sources and concentrations of carbon and nitrogen were applied to submerged culture of monascus purpureus S for understanding their effects in regulating pigment metabolites.Results showed that the carbon and nitrogen sources effected monascus purpureus S pigment significantly in its color unit and hue（p1%）.The optimal concentrations of carbon and nitrogen for generating pigment were about 4%-5% and 2.00%-2.40%,respectively.The highest color value was observed by using mannitol as only carbon source（119.6 u·g-1） and followed by rice powder（113.9 u·g-1）,the hue of both pigment products tended to red;the color value was low and the hue was towards yellow while applying glucose or sucrose as carbon source.By different nitrogen sources,the hue of pigment tended to yellow and orange-red with（NH4）2SO4 and NH4NO3,respectively,but it would tend towards red when with either one of monosodium or peptone,respectively.The color value of products was observed in order of the highest from（NH4）2SO4,then NH4NO3,with both over 100.0 u·g-1;and followed by the products from monosodium and peptone with value over 80.0 u·g-1.Results suggest that NH＋4 may enhance monascus purpureus S in generating yellow pigment while NO-3 in red pigment.