聚合回交法选育抗褐飞虱水稻恢复系

Breeding of resistance BPH restorer line using polymerization backcross

【目的】挖掘和利用稻飞虱抗性基因,选育抗褐飞虱水稻恢复系。【方法】利用高抗褐飞虱的抗源材料HS204作抗源供体,以恢复系明恢65作轮回亲本,通过回交,进行苗期群体鉴定和成株期农艺性状选择。【结果】抗褐飞虱材料HS204的抗性基因为显性。经聚合回交选育,抗褐飞虱显性基因被成功转育到轮回亲本中,从11个回交组合后代中选择获得4份配合力高、高抗褐飞虱纯合恢复系,其中M204-8-2-3-6-5、M204-8-8-14-9-9均来源于M204-8株系,其新配的7个组合抗性都在3级以上,并具有较强的恢复力。【结论】采用聚合回交法,通过抗性筛选,用抗性纯合的株系与轮回亲本回交,可加快选育目标性状纯合株系。

[Objective]The present experiment was conducted to explore and apply resistance gene of rice brown planthopper（BPH,Nilaparvata lugens Stal）to breed rice restorer with resistance to BPH.[Method]Rice material HS204 with high resistance to BPH was used as a BPH resistance donor,and rice restorer Minghui65 was used as recurrent parent.Through polymerization and backcross,the population identification of backcross offsprings at seedling stage and selection of agronomic traits at maturity were carried out.[Result]The result proved that the resistance gene of HS204 was dominant.Through polymerization and backcross,the dominant resistance gene of BPH was transferred successfully to recurrent parent.Among offspring of 11 backcross combinations,4 homozygous restorers showed high combining ability and resistance to BPH.Among 4 combinations,M204-8-2-3-6-5 and M204-8-8-14-9-9 derived from strain M204-8,and their 7 new combinations showed resistance of over grade 3 and strong restoring ability.[Conclusion]The rice homozygous strains with targeted traits can be quickly bred using polymerization backcross and resistance screening,and the homozygous resistant strains backcross with the recurrent parent.