BAK1在减弱拟南芥对Turnip crinkle virus感病性作用中的影响

Effects of BAK1 Conferring Reduced Susceptibility to Turnip crinkle virusin Arabidopsis

以Turnip crinkle virus(TCV)-拟南芥(Col-0)为互作系统,研究了跨膜蛋白BAK1是否参与拟南芥对TCV的防御作用。接种试验结果表明,bak1-4突变体对TCV更加敏感,叶片易褪绿枯萎;而过量表达植株在接种TCV后受损程度较小。H2O2检测表明,严重的病症伴随有较多活性氧积累,提示该亲和互作系统中,活性氧有利于症状的形成,BAK1对活性氧产生有负调控作用。半定量RT-PCR分析表明,BAK1过表达有利于MAPK途径下游与防御相关MPK和WRKY转录因子的激活;但不影响与病程相关水杨酸途径PR1和PR2基因的表达,它们没有介导对TCV的抗性。

An analysis was made on the effects of BAK1 in the Turnip crinkle virus （TCV）-Ara- bidopsis thaliana Col-0 compatible interaction. The results indicated that the loss of t3AK1 function led to enhanced susceptibility to infection by TCV as well as leaves＂ chlorosis and wilt. However, the plants overexpressing BAK1 delayed the disease development. H202 test showed that serious symptoms were associated with the reactive oxygen species （ROS） accumulation in this compatible interaction. BAK1 might negatively regulate ROS production. Semi-quantitative RT- PCR analysis demonstrated that the TCV-induced elevated expression of potential target genes activated transcription factors downstream of the MAPKs in BAK1 transgenic plants, which played a role in the immunity response to TCV. However, the induced PR1 and PR2 expression did not confer resistance to TCV.