猪载脂蛋白E基因的组织表达谱分析

Analysis of Tissue-specific Expression Profile of Porcine APOE

载脂蛋白E在胆固醇和其他脂质的代谢中起着至关重要的作用,对猪APOE基因的组织表达谱分析是研究该基因功能的重要基础工作。研究采用RT-PCR的方法对APOE在长白猪的13个组织中的表达进行了研究,结果显示APOE基因主要在肝和脑组织表达,其他外周组织如脾、肺、肾、大肠、小肠也有不同程度的表达,而在心、胃、背肌和淋巴中几乎不表达。

Apolipoprotein E plays an important role in cholesterol and other lipid metabolism and studying on tissue expression files of porcine APOE gene was an important basis for researching functions of the gene. In this study, RT-PCR method was used to study on expression of APOE in thirteen tissues of Landrace and the results showed that APOE gene was mainly expressed in the liver and brain tissue, and there were different expression in other peripheral tissues such as spleen, lung, kidney, large intestine, small intestine, whereas almost no expression in heart, stomach, M. longissimus and lymph in pig.