铅对小鼠睾丸生精细胞凋亡及Caspase-3,Bcl-2和Bax基因表达的影响

Effects of Lead on Testicular Cells Apoptosis and Expression of Caspase-3,Bc-2 and Bax Genes in Mouse

为了研究铅致小鼠睾丸生精细胞凋亡及其对Caspase-3,Bax和Bcl-2基因表达的影响.25只雄性小鼠随机分成5组:0h对照组、12h染铅组、24h染铅组、36h染铅组和48h染铅组,每组5只,实验组按照100mg/kg的剂量腹腔注射醋酸铅溶液,5组小鼠分别于0h、12h、24h、36h、48h后脊柱拉断处死,取其睾丸组织,用苏木精-伊红染色及免疫组化技术检测睾丸组织细胞凋亡及凋亡相关蛋白Caspase-3,Bcl-2和Bax的表达.结果显示:细胞凋亡相关基因Caspase-3,Bcl-2和Bax的表达较对照组差异显著,Caspase-3和Bax在染铅毒后表达量一直上升,Bcl-2的表达量一直下降,差异性随染铅时间的不同而有变化.铅负荷至一定程度时可致小鼠睾丸细胞凋亡,且呈一定量效关系(P<0.01),这种作用可能与Bcl-2基因低表达和Caspase-3与Bax基因高表达有关.

To investigate the occurrence of testicular cell apoptosis and the expression of the apoptosis related gene Caspase-3, Bcl- 2 and Bax in the adult male mice following lead administration, twenty five adult mice were randomly divided into blank control group （Oh） and 12h, 24h, 36h, 48h lead exposed group （five mice in each group） and treated with lead acetate solution （100mg/kg） by intraperitoneal injection. The mice were sacrificed at Oh, 12h, 24h, 36h and 48h respectively and testes were isolated. The testes were then fixed and processed for hematoxylin - eosin and immunohistochemical staining to detect cell apoptosis and the level of Caspase - 3, Bcl - 2 and Bax gene expression. Compared with the control group, the protein level of Bcl - 2 was significantly lower （P〈 0.01 ）, whereas the protein levels of Caspase - 3 and Bax were significantly higher （P〈 0.01） in experimental groups. The degree of differences was correlated with the time of lead exposure. The testicular apoptotic cell death is highly associated with lead loading and changes in Caspase - 3 and Bcl - 2/Bax expression may play an important role in this process.