鲤四种经济性状的微卫星标记分析

Analysis on four economical traits in common carp(Cyprinus carpio L.) by microsatellite markers

获得与经济性状相关的分子标记或基因是开展该性状分子育种的先决条件。本文利用300个微卫星标记检测了以柏氏鲤和荷包红鲤抗寒品系杂交F2代1个家系、92个个体的基因型,回归分析了其体长、体厚、体高和体重4种经济性状的GLM单标记。Permutation检验结果显示:有23个标记分别与体长、体厚、体高和体重具有显著相关性(P<0.05),其中,HLJ44、HLJE28、HLJ1148、HLJ1329、HLJ464与这4种性状极显著相关(P<0.01)。对同一标记不同基因型之间进行了多重比较,找到了与这4种性状相关的基因型。此研究还发现75个微卫星标记发生了偏分离,其中11个偏分离标记与性状显著相关,偏分离指数分析表明,基因型的偏分离可能与性状的选择压力和选择方式有关。本研究获得的与鲤4种性状相关的标记及基因型,为鲤的分子标记辅助育种(MAS)和生长性状的遗传机制研究提供了有效的依据。

The prerequisite of marker-assisted selection（MAS） is to find molecular markers or genes associated with those economic traits which are mostly controlled by a number of genes and inherited as quantitative traits.In this study,300 microsatellite markers were used to analyze the genotypes in the reference panel contained 92 individuals in a F2 family obtained by mating of F1 hybrids derived from crossing Barbless common carp（Cyprinus pellegrini） with Hebao？cold tolerance red common carp（Cyprinus carpio wuyuanensi）,and four economic traits including standard length,body width,body depth and body weight were tested using GLM（General Linear Model） single marker of the regression.The determination of the threshold values by 10000 Permutation test showed that 23 markers had significant correlation（P 0.05） with the four traits,in which HLJ44,HLJE28,HLJ1148,HLJ1329,HLJ464,and HLJE469 had very significant correlation with the standard length,body width,body depth and body weight（P 0.01）.In addition,the genotypes of these correlative loci captured were determined by Daucan＇s in SPSS13.0 software,and 75 segregation distorted microsatellites were detected whereas 11 segregation distorted microsatellites had correlation with the traits.Genotype deviate index describes the content of those genotypes deviation,the possibility of deviated genotypes linked with selection pressures and selection methods.These functional markers and genotypes related to the traits may be used for marker assisted selection and provided the efficient evidence of genetic mechanism on growth traits in common carp.