骨巨细胞瘤瘤组织中M-CSF、TNF-α的检测及其意义

THE DETECTION AND ITS SIGNIFICANCE OF M-CSF AND TNF-α IN THE TUMOR TISSUE OF GIANT CELL TUMOR OF BONE

目的 :探讨细胞因子与骨巨细胞瘤局部溶骨的关系。方法 :通过ELISA法、Westernblot法和免疫组化法对 10例骨巨细胞瘤、5例骨肉瘤和 5例正常人血清进行了TNF -α和M -CSF表达及定位检测。结果 :骨巨细胞瘤组织TNF -α含量和M -CSF表达率明显高于骨肉瘤和正常人血清。TNF -α由骨巨细胞的部分单核基质细胞和多核巨细胞分泌 ;而M -CSF由部分单核基质细胞分泌 ,多核巨细胞则不表达。结论

Objectives:To study the relationship between some cellular factors and local osteolysis of giant cell tumor of bone. Methods:In 10 cases of giant cell tumor of bone,5 cases of osteosarcoma and 5 cases of normal control serum, the expression and positioning detection of THF-α (tumor necrosis factor) and M-CSF were performed by ELISA,Western Blot and immunohistochemical method.Results:The content of TNF-α and the expression rate of M-CSF in the tissue of giant cell tumor of bone were significantly higher than that in osteosarcoma and normal control serum. The TNF-α was exereted by partial uninuclear matrix cells and coenocytic giant cells of the giant cells of bone, but the M-CSF was just excreted by partial uninuclear matrix cells. Conclusions:Different cellular factors excreted by different cells in giant cell tumor of bone may involve in local bone absorption.