铜和锌对小麦胚芽愈伤组织维生素E积累的影响(英文)

Effect of Copper and Zinc on Accumulation of Vitamin E in Wheat Embryo-derived Callus

[目的]考查培养基中铜元素和锌元素的含量对小麦胚芽愈伤组织维生素E积累的影响。[方法]通过建立维生素E积累和细胞生长的数学模型,描述小麦胚芽愈伤组织的维生素E积累和细胞增长。以次生代谢产物维生素E积累最多为目标,通过试验优化确定铜锌含量的最佳组合。[结果]结果表明,当B5培养基中ZnSO4.7H2O为2.0mg/ml;CuSO4.5H2O为0.1mg/ml时,有利于维生素E积累。维生素E积累的动力学模型的拟合度为97.53%,细胞干重的动力学模型拟合度为95.60%,非线性关系良好。[结论]微量元素铜和锌对小麦胚芽愈伤组织维生素E积累有影响,铜元素较锌元素的影响显著,且低浓度的铜锌之间有协同作用;随着浓度增大,抑制作用逐渐增强。

[Objective] The aim was to study the effect of the content of copper and zinc on in medium the vitamin E accumulation in wheat embryo-dreived callus.[Method] The mathematical models were established to describe the growth kinetics and the vitamin E accumulation in wheat embryo callus cells.With the aim of getting the highest accumulation of the secondary metabolite Vitamin E,the optimal combination of copper and zinc in medium was confirmed by testing.[Result] The results showed that the production of vitamin E in B5 medium reached the highest value with 2.0 mg/mL ZnSO4·7H2O and 0.1 mg/mL CuSO4·5H2O.The fitting degrees of kinetic models of vitamin E accumulation and cell growth were 97.53% and 95.60%,respectively,which indicated good nonlinear relationships.[Conclusion] Both copper and zinc could affect the accumulation of vitamin E in wheat germ callus,and Copper showed more prominent effect than Zn.Synergism existed in low copper and zinc concentration,and the inhibitive effect enhanced with the increase of the concentrations.