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Fasudil促进大鼠损伤脊髓神经功能恢复的研究 被引量：3

Effect of Fasudil on recovery of neuro-function after spinal cord injury(SCI)in rats
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摘要 目的研究Rho激酶抑制剂Fasudil对大鼠脊髓损伤的促修复作用。方法用健康成年SD大鼠建立脊髓损伤模型,通过腹腔注射盐酸法舒地尔,并设立对照。术后1、2、4周,运用BBB功能评分进行后肢运动功能评价;损伤后4周,脊髓损伤局部行SABC法ROCK2免疫组织化学染色。结果术后1、2、4周,Fasudil治疗组与对照组比较,评分明显增高(P<0.05);术后4周Fasudil治疗组损伤局部组织内ROCK2的表达较对照组明显减少(P<0.05)。结论腹腔注射Fasudil能够减少脊髓损伤局部ROCK2表达,改善大鼠的运动功能。 Objective To investigate the effect of Fasudil as a selective Rho kinase inhibitor on recovery of neuro-function after spinal cord injury（SCI）in rats.Methods The female Sprague-Dawley（SD）rats of SCI were made by hemi-section of spinal cord.The model rats were randomly divided into two groups：Fasudil treatment group and control group.Hindlimbs functional recovery was evaluated by BBB scales at 1st week,2nd week and 4th week after operation.The expression of Rho-kinase 2（ROCK2）in the injured spinal cord was detected by immunohistochemical method at 4th week after operation.Results The BBB scores of Fasudil treatment rats increased significantly than those of control rats at 1st week,2nd week,and 4th week after operation,respectively（P0.05）.The expression of ROCK2 in the injured spinal cord of Fasudil group decreased significantly than that of control group at 4th week（P0.05）.Conclusion Fasudil can reduce the expression of ROCK2 in the injured spinal cord,and improve the locomotor function of rats.
作者 辛国强 袁绍纪 卢培刚
机构地区 济南军区总医院神经外科
出处 《实用药物与临床》 CAS 2011年第6期454-456,F0003,共4页 Practical Pharmacy and Clinical Remedies
关键词 脊髓损伤 ROCK2 法舒地尔 Spinal cord injury ROCK2 Fasudil
分类号 R651.2 [医药卫生—外科学]
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参考文献5

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同被引文献33

  • 1肖保国.Rho激酶抑制剂的研究现状及其在神经系统疾病中的应用前景[J].重庆医科大学学报,2008,33(z1):99-100. 被引量：11
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  • 3Ryan T J, Grant S G. The origin and evolution of synapses[J]. Nat Rev Neurosci, 2009 , 10:701-712.
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  • 9Wang Y X, Martin-Mc Nulty B, Cunha V et al. Fasudil, a Rho-ki- nase inhibitor, attenuates angiotensin Ⅱ-induced abdominal aortic aneurysm in apolipoprotein E-defident mice by inhibiting apoptosis and proteolysis[ J]. Circulation, 2005 , 111 : 2219-2226.
  • 10Rikitake Y, Kim Y Y, Huang G et al. Inhibition of Rho kinase (ROCK) leads to increased cerebral blood flow and stroke protection [J]. Stroke, 2005, 36(10):251-257.

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实用药物与临床
2011年 第6期

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