乙醇提取柚子皮中总黄酮条件及其抑菌分析

Study on the processing condition and bacteriostasis analysis of extracting total flavonoids from shaddock peel

对乙醇提取柚子皮中总黄酮的影响因素作了探讨,确定了乙醇提取黄酮的条件为:水浴温度70℃、乙醇体积浓度50%、提取时间2.0 h、固液比1 g∶25 mL,该条件下测得总黄酮的提取率为4.39%。该提取物对大肠杆菌有一定的抑制效果,对酵母菌几乎没有抑制作用。

The influence factors of total flavones in Shaddock Peel with alcohol were discussed. The result showed that the extraction rate of total flavonoids reached 4.39% at the optimal processing condition： extraction temperature 70 ℃, extraction time 2 h, alcohol concentration 50%, solid-liquid ratio 1 ： 25. The conclusion was the extraction can inhibit escherichia coli（e.coli）and can hardly inhibit yeast.