赤潮毒素大田软海绵酸表面等离子共振免疫检测方法研究

Research of a SPR Immunoassay Method for Okadaic Acid Contamination of Red Tide Toxins

结合表面等离子体共振技术与免疫检测技术,研究和建立了一种响应速度快、免标记、低成本新型海洋赤潮毒素大田软海绵酸检测方法。基于SpreetaTM传感器构建了小型表面等离子共振免疫检测系统,采用共价偶联方法在传感器金膜表面修饰大田软海绵酸-牛血清蛋白抗原作为生物敏感膜;测得该方法相对标准偏差为1.51%(n=12),定量范围为40 ng/mL～640 ng/mL,检测限为19.4 ng/mL,半抑制浓度IC50为177 ng/mL。结果表明该方法具有较好的重复性;所设计的检测芯片可重复使用,节省了分析测试的成本。该方法对实现赤潮毒素的现场分析有较大的应用前景。

A novel assay method for okadaic acid（OA）of red tide toxins has been exploited,which employed surface plasma resonance（SPR）technique and immunoassay method.SpreetaTM sensor was adopted in the SPR flow injection analysis（FIA）system.The reusable immunosensor chip was functionalized with okadaic acid-bovine serum albumin（OA-BSA）using covalent-coupling.Acceptable repeatability was achieved with relative standard deviation（RSD）1.51%（n=12）.Calibration curve obtained for OA has detection limit of 19.4 ng/mL,half maximal inhibitory concentration（IC50）of 177 ng/mL and quantitative range of 40 ng/mL^640 ng/mL.The method owns a desirable repeatability.Using self-designed reusable biochip,it has an advantage of cost-effectiveness.The method has a good prospect of on-site determination of OA.