摘要
目的建立高效液相色谱(HPLC)法测定参麻通络胶囊中芍药苷含量。方法色谱柱为Di-amonsil,C18,5μm,4.6mm×250 mm,甲醇-水-冰醋酸(32∶68∶0.2),流速为1.0 mL/min,检测波长为230 nm。进样量为20μL。结果芍药苷在0.0071~0.0427 mg/mL范围内呈良好的线性关系。平均回收率为99.51%。结论本品方法操作简便、可靠,重现性好,专属性强,可作为参麻通络胶囊的内在质量控制方法。
Objective To establish a method for Determination of paeoniflorin content in Shenma Tongluo capsule by High-performance liquid chromatography(HPLC).Methods Chromatographic conditions of paeoniflorin determination are as follows: column(Diamonsil,C18,5 μm 4.6 mm×250 mm),mobile phase [Methanol-water-glacial acetic acid(32∶68∶0.2)],flow(1.0 mL/min),detection wavelength(230 nm) and sample volume(20 μL).Results Paeoniflorin in 0.007 1 ~ 0.042 7 mg/mL range was a good linear relationship.The average recovery was 99.51%.Conclusion The method is simple,reliable,reproducible,specific,and can be used as intrinsic quality control of Shenma Tongluo capsule.
出处
《河北中医》
2011年第10期1548-1550,共3页
Hebei Journal of Traditional Chinese Medicine
关键词
回收率
丹参
天麻
通络
胶囊
色谱法
高压液相
芍药甙
Recovery
Salvia
Gastrodia
Meridians
Capsule
Chromatography
High-pressure liquid
Paeoniflorin