滚环扩增技术及其在生物分子诊断中的应用

Rolling Circle Amplification and Its Application in bio-molecular Diagnosis

滚环扩增技术(RCA)是近年来发展起来的一种新型的核酸扩增技术。该技术是基于连接酶连接、引物延伸、与链置换扩增反应的一种等温核酸扩增方法。在恒温的条件下,可以产生大量的与环型探针互补的重复序列。与传统的核酸扩增方法相比,它具有扩增条件简单,特异性高,能在恒温条件下进行等特点。滚环扩增技术结合荧光、电化学、电化学发光等检测技术可以实现高灵敏的生物分子检测。这些特点使得滚环扩增技术在生物分子诊断领域具有潜在的应用价值。本文概述了滚环扩增技术的基本原理,详细地介绍了滚环扩增技术在当前生物检测中的应用现状,并对其前景作了展望。

Rolling circle amplification（RCA） is a new type of nucleic acid amplification technology developed in recent years.It is based on the amplification of a circularizable oligonucleotide probe（C-probe）,which is ligated bv DNA ligase.Under isothermal condition,C-probe can be amplified and can generate multimeric single-stranded DNA（ssD-NA） complementary to C-probe.Compared with traditional nucleic acid amplification method,RCA is a simpler amplification method with high specificity.Combined with fluorescence detection,electrochemical detection,electrochemiluminescence detection,et al,RCA can be applied to high sensitive analysis of bio-molecules.Due to the fact that RCA can be carried out under constant temperature conditions,this feature make RCA hold great potential in the field of point-of-eare diagnosis.This article outlines the basic principles of RCA,the detailed description of the current status of rolling circle amplification in biological detection,and its future prospects.