正交设计在根茎秋海棠‘Helen Lewis’组织培养中的应用

Application of orthogonal design in tissue culture of Begonia Helen Lewis

用正交试验设计法研究了6-BA,NAA和24,-D 3种植物生长调节剂对根茎秋海棠‘Helen Lewis’叶片诱导不定芽的影响,建立了根茎秋海棠叶片再生体系。结果表明,诱导根茎秋海棠不定芽发生的最佳配方为:MS+2 mg.L-1 6-BA+0.5 mg.L-1 NAA+0.5 mg.L-1 2,4-D,36 d时诱导率为86.7%,并有淡绿色愈伤组织形成。附加0.5 mg.L-1 6-BA+0.2 mg.L-1 NAA的MS培养基有利于不定芽的分化和增殖。在1/2 MS+0.5 mg.L-1 IBA的培养基中生根效果最好,高于4 cm的再生植株移栽成活率达90%以上。

Effects of 6-BA,NAA and 2,4-D on adventitious buds induction from leaf explants of Begonia Helen Lewis were studied by L9（34）orthogonal design.In the present work we established an in vitro regenerative protocol for the large-scale propagation.The experimental results showed that the best medium for adventitious buds induction was MS with 2 mg·L-1 6-BA,0.5 mg·L-1 NAA,0.5 mg·L-1 2,4-D,and young leaves used as explants showed 86.7% induction rate of adventitious buds for about 36 d,with light green calli formed.The MS media supplemented with 0.5 mg·L-1 6-BA and 0.2 mg·L-1 NAA was beneficial to differentiation and proliferation of adventitious buds and calli,while the best rooting effect was in 1/2 MS medium with 0.5 mg·L-1 IBA.Plantlets longer than 4 cm were successfully transplanted into soil medium showing a survival rate of 90%.