HPLC和SOS显色反应检测不同微生物脱除4-NQO基因毒性能力

Determination of Antigenotoxic Activity of Different Probiotic Microorganisms against 4-Nitroquino-line-1-oxide(4-NQO) by SOS Chromotest and HPLC

应用高效液相色谱法(high performance liquid chromatography,HPLC)和SOS显色反应测定36株益生菌脱除4-硝基喹啉-1-氧化物(4-NQO)基因毒性的能力,同时用HPLC对它们的图谱进行比较分析。研究发现,基因毒性清除率高的菌株有1株植物乳杆菌、5株双歧杆菌、干酪乳杆菌代田株、3株芽孢杆菌、2株酵母菌、7株唾液乳杆菌和一株保加利亚乳杆菌。同属于一个菌属的各个菌株基因毒性清除率之间差异不显著。图谱分析发现,只有唾液乳杆菌才产生P1,即益生菌脱除4-NQO基因毒性具有菌株特异性。研究还发现益生菌转化4-NQO为4-HAQO的能力是决定益生菌脱除4-NQO基因毒性能力大小的关键。

SOS chromotest and HPLC were used for determining the antigenotoxic activity of 36 probiotic strains against 4-NQO. Meanwhile, their ability to remove 4-NQO genotoxicity was comparatively analyzed by HPLC. One L. plantarum strain, 5 Bifidobacterium strains, L. casei strain Shirota, 3 Bacillus strains, 2 yeast strains, 7 Lsalivarius strains and I Lactobacillus bulgaricus strain were found to have a higher ability to remove 4-NQO genotoxicity among these strains. No significant difference in removal ability for 4-NQO genotoxicity was observed among strains from the same genus. Only L.salivarius strains could produce Px, suggesting the species-specific removal ability of probiotics for 4-NQO genotoxicity. Also, we found that the capability of converting 4-NQO into 4-HAQO decided the ability of probiotics to remove 4-NQO genotoxicity. This study can provide references and supports for further studies of the removal mechanism of 4-NQO genotoxicity by probiotics.