颐脑解郁方对抑郁模型大鼠脑内GFAP、bFGF、BDNF的影响

Influences of Yinao Jieyu Fang on brain GFAP,bFGF and BDNF in rat model of depression

目的研究颐脑解郁方对抑郁模型大鼠脑内胶质原纤维酸性蛋白(GFAP)、碱性成纤维细胞生长因子(bFGF)及脑源性神经营养因子(BDNF)表达的影响。方法二级雄性Wistar大鼠分为正常组、抑郁组、治疗组、对照组。抑郁组、治疗组、对照组采用慢性应激方法结合孤养法造成抑郁大鼠模型,治疗组每日灌服中药颐脑解郁方1次,连续给药6周,对照组每日灌服生理盐水1次,连续6周。取脑组织,采用免疫组化方法,测定各组大鼠脑额叶皮质及海马GFAP、bFGF、BDNF的表达。结果对照组大鼠脑额叶皮质及海马GFAP、bFGF、BDNF阳性细胞数量减少,表达减弱,细胞大小不均,排列不规则。治疗组大鼠脑额叶皮质及海马GFAP、bFGF、BDNF阳性细胞数目显著增加,形态规则,表达增强;对GFAP、bFGF、BDNF阳性细胞进行测量分析,统计每个场的面密度,与对照组相比,有统计学意义(P<0.05或P<0.01)。结论颐脑解郁方通过增强模型大鼠脑内GFAP、bFGF、BDNF的表达,促进5-羟色胺、去甲肾上腺素的功能,从而改善抑郁症状。

Objective To study the influences of Yinao Jieyu Fang on the expressions of brain glial fibrillary acidic protein （GFAP）, basic fibroblast growth factor （bFGF） and brain-derived neurotrophic factor （BDNF） in rat model of depression. Methods Male Wistar rats were divided into the normal group, depression group, treatment group and control group. The depression model was established by using chronic stress and independent feeding in the depression group, treatment group and control group. Then the treatment group was intragastrically given Yinao Jieyu Fang once a day for 6 weeks, and control group, normal saline solution once a day for 6 weeks. The expressions of GFAP, bFGF and BDNF in rat frontal cortex and hippocampus were detected by applying immunohistochemistry assay. Results The number of GFAP, bFGF and BDNF decreased, and their expressions were reduced and shapes were different in size with irregular arrangement in the control group. The number of positive cells of GFAP, bFGF and BDNF increased significantly, and their shapes were regular and expressions were improved in the treatment group. The positive cells of GFAP, bFGF and BDNF were measured and analyzed and every area density was counted, and the difference was statistically significant compared with the control group （P 〈 0. 05 or P 〈 0. 01 ）. Conclusion Yinao Jieyu Fang can ameliorate depression symptomsthrough improving the expressions of GFAP, bFGF and BDNF, and enhancing the function of 5-hydroxytryptamine （5-HT） and norepinephrine （NE）.