交变电场对过氧化氢酶活性影响的研究

Study on the Effects of Alternate Electric Field on the Activity of Catalase

过氧化氢酶能够催化植物光呼吸、线粒体电子传递及脂肪酸β-氧化等过程中产生的过氧化氢,生成氧气,从而消除其对植物细胞产生的危害.本研究采用钼酸铵法,以绿豆真叶过氧化氢酶粗提液为研究对象,用交变电场处理,研究过氧化氢酶在不同电场下的活性.实验过程中分别选用不同电场频率、不同峰值电压以及不同的电场处理时间,研究其对过氧化氢酶活性的影响.结果表明,电场处理过氧化氢酶能构不同程度的提高过氧化氢酶的活性,且在频率为5MHz,电压为600mV,处理时间为5min条件下酶活性达到理想值,酶活为1.99mmol/(min.g).

The hydrogen peroxide plant enzyme could catalyze photorespiration, mitochondrial electron transport and fatty acid β--oxidation process of hydrogen peroxide produced, generating oxygen, thereby eliminating the hazards of plant cells. In this study, ammonium molybdate method, in order to mung bean Leaf catalase in crude extracts was studied using alternating electric field treatment, research catalase activity in different electric field. The experiment, respectively, the alternative electric field frequency, peak voltage and the electric field of different processing time to study the activity of hydrogen peroxide effects. The results show that electric field treatment on catalase structure can be improved to varying degrees of catalase activity,and in the frequency of 5 MHz,voltage of 600 mV,proeessing time is 5 min activity under the conditions to achieve the desired value,the activity of 1.99 mmol / （min . g）.