高效液相色谱法测定肝得宁丸中五味子甲素的含量

Content Determination of Schiandrin A in Gandening Pill by High Performance Liquid Chromatography

目的建立肝得宁丸中五味子甲素含量测定方法。方法采用高效液相色谱法,色谱柱:phenomenex Luna C18(5μm,250 mm×4.6 mm);流动相:乙腈-水-醋酸(64:35:1);流速:1 ml/min;检测波长:254 nm;柱温25℃。结果五味子甲素含量在0.2624～0.5248μg范围内线性关系良好,平均回收率为98.91%,相对标准偏差(RSD)为0.84%。结论该方法操作简便,准确可靠,专属性强,重复性好,可用于肝得宁丸的质量监控。

Objective To establish a method for determining schiandrin A in Gandening Pill.Methods The high performance liquid chromatography was used to dectect the content of schiandrin A.The chromatography column was a phenomenex Luna C18（5 μm,250 mm×4.6 mm）.The mobile phase was acetonitrile-water-ace acid（64：35：1）;flow velocity was 1 ml/min;detective wavelength was 254 nm and column temperature was set at 25℃.Results Good linearity was obtained in the range of 0.2624-0.5248 μg,and the recovery and RSD were 98.91% and 0.84% respectively.Conclusion The method is accurate,simple,and reliable with good specificity and repeatabicity for quality control of Gandening Pill.