期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

花生中两个CBF-like基因全长的克隆与序列分析 被引量：2

Clone and Sequence Analysis of Two CBF-like Genes in Peanut
下载PDF
导出
摘要 DREB/CBF类转录因子的功能及作用机理在拟南芥、水稻等模式植物中已经得到了较为充分的研究,但是在花生中还没有关于这类基因的报道,其功能也没有得到证实。本研究通过Bi-oedit软件在花生cDNA文库中找到了15个可能编码CBF类蛋白的基因片段,其中有2个基因没有包含完整的开放阅读框。我们通过5'-RACE RT-PCR对这两个基因的全长进行了克隆,得到了包含完整读码框的基因序列,并将序列在GenBank上注册,同时对得到的序列进行了初步分析。 The function of DREB/CBF transcript factors had been extensively studied in Arabidopsis and rice. However, no researches had been done in peanut about this kind of gene. In this study, we found 15 ESTs which may encode DREB/CBF proteins from the cDNA library of peanut using the Bioedit software. 5＇ fragments of 2 ESTs were unknown and we amplified the 5＇-end of these two genes through 5＇--RACE RT--PCR. The sequences of the two genes were also submitted to Gen- Bank and analyzed after the whole sequences were cloned.
作者 陈娜 程果 胡东青 杨庆利 迟晓元 潘丽娟 杨珍 陈明娜 和亚男 禹山林
机构地区 山东省花生研究所 青岛市出入境检验检疫局
出处 《花生学报》 2011年第4期1-8,26,共9页 Journal of Peanut Science
基金 山东省自然科学基金项目(ZR2011CQ036 ZR2009DQ004) 国家自然科学基金项目(31000728)
关键词 CBF—like基因 花生 基因克隆 序列分析 CBF-like gene peanut （Arachis hypogaea L. ） gene clone sequence analysis
分类号 S565.2 [农业科学—作物学] Q785 [生物学—分子生物学]
  • 相关文献

参考文献23

  • 1Bohnert H J, Ayoubi P, Borchert C, et al. A genomic approach towards salt stress tolerance [ J]. Plant Physiol Biochem, 2001, 39:295--311.
  • 2Seki M, Narusaka M, Abe H, et al. Monitoring the expression pattern of 1300 Arabidopsis genes under drought and cold stresses by using a full-length cDNA microarray[J]. Plant Cell, 2001, 13:61--72.
  • 3Zhu T, Budworth P, Han B, et al. Toward elucidating the global expression patterns of developing Ara- bidopsis: parallel analysis of 8300 genes by a high- density oligonucleotide probe array[J]. Plant Physiol Biochem, 2001, 39 : 221-- 242.
  • 4Pradeep K. Agarwal, Parinita Agarwal, M. K. Red- dy, et al. Role of DREB transcription factors in abi- otic and biotic stress tolerance in plants [J]. Plant Cell Rep, 2006, 25. 1263--1274.
  • 5Stockinger EJ, Gilmour SJ, Thomashow MF. Arabi- dopsis thaliana CBF1 encodes an AP2 domain-contai- ning transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low tempera- ture and water deficit[J]. Proc Natl Acad Sci USA, 1997, 94:1035--1040.
  • 6Liu Q, KasugaM, Sakuma Y, Abe H, et al. Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought-and low-temperature-responsive gene expression, respectively, in Arabidopsis[J]. Plant Cell, 1998, 10:1391 --1406.
  • 7Jaglo KR, Kleff S, Amundsen KL, et al. Components of the Arabidopsis C-repeat/dehydration-re- sponsive element binding factor cold-response pathway are conserved in Brassica napus and other plant species[J]. Plant Physiol, 2001, 127:910--917.
  • 8Park JM, Park CJ, Lee SB, et al. Overexpression of the tobacco Tsil gene encoding an EREBP/AP2-type transcription factor enhances resistance against pathogen attack and osmotic stress in tobacco [ J]. Plant Cell, 2001, 13:1035--1046.
  • 9Dubouzet JG, Sakuma Y, Ito Y, et al. OsDREB genes in rice, Oryza sativa L. , encode transcription activators that function in drought-, high-salt-and cold-responsive gene expression[J]. Plant J. , 2003, 33:751--763.
  • 10Li XP, Tian AG, Luo GZ, et al. Soybean DRE- binding transcription factors that are responsive to abiotic stresses[J]. Theor Appl Genet, 2005, 110: 1355--1362.

二级参考文献9

共引文献135

同被引文献15

引证文献2

二级引证文献4

花生学报
2011年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部