摘要
通过体外耐酸、耐胆盐和抗氧化活性分析法研究了猪肠道来源的5株乳杆菌的潜在益生特性。结果表明,5株乳酸杆菌均能耐受pH3.0。菌株PCL 3对pH 2.0具有良好耐受能力。受试菌株对0.3%~1.0%的牛胆盐具有良好的耐受能力,1%（w/v）胆盐处理4h,所有受试菌株的存活率均高于80%。抗氧化活性分析表明,菌株具有一定的体外清除羟自由基和DPPH自由基能力。
Five strains of lactobacilli which were isolated from the gastrointestinal tract of pig were evaluated for probiotic potential with the pH and bile salts tolerance,and antioxidative activities.The results showed that all strains grew well at acid condition（pH 3.0,3h of incubation）.Among 5 lactobacilli strains,PCL3 showed good tolerance to pH 2.0 for 3h.All strains could survive in the medium at 0.3%-1% bile salts.PCL3 possessed the highest ability of bile salts tolerance.Bioactivity tests in vitro showed that the all strains displayed ability to scavenge hydroxyl and DPPH radicals.
关键词
乳杆菌
益生性
耐受
抗氧化
lactobacilli
probiotic
tolerance
antioxidative
