四黄汤对急性肝损伤小鼠保护作用的研究

Protective Effect of Sihuangtang on Acute Hepatic Injury in Mice

目的:探讨四黄汤对小鼠急性肝损伤的保护作用。方法:将60只小鼠随机分为四组,正常对照组15只、模型组15只、四黄汤高剂量组15只(40 g/kg/d生药)及四黄汤低剂量组15只(20 g/kg/d生药)。模型组每天灌以相同容积的生理盐水,连续2周。最后一次灌胃24 h后,除正常对照组外,所有小鼠以CCl4(0.1%10 mL/kg)腹腔注射,禁食不禁水8 h后断头取血及肝脏,检测血清谷丙转氨酶(ALT)的活性;部分肝组织制备肝匀浆,检测丙二醛(MDA)的含量,同时取相同部位的肝组织制备病理切片,观察小鼠肝组织损伤的程度。结果:急性肝损伤模型组血清ALT的含量明显高于正常对照组(P<0.05);四黄汤低剂量干预组血清ALT的含量则低于模型组(P<0.05),而高剂量干预组血清ALT含量则较模型组偏高(P<0.05);模型组肝组织MDA含量高于正常对照组(P<0.05);四黄汤低剂量及高剂量干预组肝组织MDA的含量低于模型组(P<0.05)。光镜下四黄汤低剂量干预组肝细胞损伤程度较急性肝损伤模型组减轻。结论:四黄汤对小鼠的急性肝损伤具有一定的保护作用。

Objective： To investigate the protective effect of Sihuangtang on acute hepatic injury in mice and its mechanism.Methods： Sixty mice were randomly divided into 4 groups each with 15,normal control group,model group,Sihuangtang high-dose group（40 g/kg/d crude）,and Sihuangtang low-dose group（20 g/kg/d crude）.Drug administration was conducted with gastric lavage in different dose everyday for 2 weeks in two treated groups,the same volume of normal saline was applied for model group.Twenty four hours after last gastric lavage,except normal control group,all mice were fasting for 8 hours and then euthanized with intraperitoneal injection of CCl4（0.1% 10 mL/kilogram）.The levels of alanine aminotransferase（ALT） in the serum,and malonaldehyde（MDA） in the liver were determined.And the pathological examination of liver tissue was observed by optical microscope.Results： The MDA level in the liver and the content of ALT in the serum of acute hepatic injury model group were significant higher than those in normal control group（P〈0.05）;compared with the acute hepatic injury model group,the MDA levels in the liver in Sihuangtang low-dose and high-dosage group were both evidently decreased（P〈0.05）;and the content of ALT in the serum of Sihuangtang low-dosage group was evidently decreased compared with the model group（P〈0.05）,but the content of ALT in the serum increases in Sihuangtang high-dose group compared with the model group（P〈0.05）.Under the optical microscope,liver cell injury of the low dose group was evidently slighter than that of the model group.Conclusion： Sihuangtang in certain concentration has protective effect on acute hepatic injury in mice.