灵芝菌液态发酵富硒大豆过程中酶活性的研究

Study on the Enzyme Activity of Liquid-state Fermentation of Selenium-Enriched Soybean by Ganoderma lucidum Mycelium

以富硒大豆豆乳为培养基,接种灵芝菌种进行发酵,对发酵过程中的酸性蛋白酶、中性蛋白酶、淀粉酶、内切β-1,4-葡聚糖酶、外切β-1,4-葡聚糖酶、β-葡萄糖苷酶活力变化趋势进行研究.结果表明,在0～132 h范围内,酸性蛋白酶、淀粉酶、内切β-1,4-葡聚糖酶、外切β-1,4-葡聚糖酶、β-葡萄糖苷酶活力呈增加趋势,发酵至132 h,酸性蛋白酶、淀粉酶、内切β-1,4-葡聚糖酶、外切β-1,4-葡聚糖酶、β-葡萄糖苷酶活力达最大值,分别为9.87、0.87、0.951、.07、1.85 U/mL,中性蛋白酶活力在发酵108 h达最大值5.35 U/mL.

The change tendencies of acidic proteinase activity,neutral proteinase activity, amylase activity, endo-glucanase activity, exo-glucanase activity and β -glucosidase activity were studied during liquid-state fermentation of Ganoderma lucidum by using selenium-enriched soybean emulsion as medium. The results showed that the acidic proteinase activity, amylase activity, endo-glucanase activity, exo-glucanase activity and β -glucosidase activity increased during liquid-state fermentation of Ganoderma lucidum. The enzylne activity reached the highest degree in 132 h in fermentation, the acidic proteinase activity 9.87 U/mL, amylase activity 0.87 U/mL, endo-glucanase activity 0.95 U/mL, exo-glucanase activity 1.07 U/mL, β-glucosidase activity 1.85 U/mL. The neutral proteinase activity reached the highest degree 5.35 U/mL in 108 h.