不同产地苦参饮片水煎液中三叶豆紫檀苷含量测定

Determination Content of Trifolirhizin in Sophora flavescens Herbal Pieces Decoction from Different Areas

目的:建立苦参饮片水煎液中三叶豆紫檀苷含量测定方法。方法:采用HPLC法,Alltech Apollo C1(84.6 mm×150 mm,5μm)为色谱柱,以乙腈-水(30∶70)为流动相,流速:1.0 mL.min-1,检测波长:310 nm,柱温:25℃。结果:三叶豆紫檀苷回归方程Y=676.08X+7.5(r=0.9994),三叶豆紫檀苷在0.982-4.64μg线性关系良好;方法的平均回收率为99.09%,RSD%=1.31%。结论:所建立的方法准确、灵敏、重复性好,可用于考察不同产地苦参饮片水煎液中主要黄酮成分含量。

Objective：To establish the determination method of trifolirhizin content in Sophora flavescens herbal pieces decoction and to investigated the major flavonoid components in Sophora flavescens herbal pieces decoction. Method： Alltech Apollo C,s （4.6 mm×150 mm,5μm）eolmnn was used,the mobile phase was aeetonitrile-water（30：70）;with the flow rate as 1.0 mL·min^-1,the detection wavelength was at 310 nm. Result：The linear relation of hyperin was good in the range from 0.982 to 4.64 μg.The average recovery was 99.09%,RSD 1.31%. Conclusion：The method established is accurate,sensitive and good in reproducibility. It can be applied to the quality control of Sophora flaveseens Herbal Pieces.