响应面法优化益母草中总生物碱提取工艺

Optimization of the extraction technology of total alkaloid in motherwort with response surface methodology

目的:优化益母草中总生物碱提取工艺。方法:采用单因素考察和响应面分析法相结合的方法,对乙醇浓度、提取时间、乙醇体积3个影响益母草中总生物碱提取率的关键因素进行优化分析。结果:建立了益母草中总生物碱提取模型,模型R2=0.99512,回归模型显著;提取优化条件:乙醇浓度为96%,提取时间为2.7 h,乙醇体积352 mL。益母草中总生物碱的提取率为0.186%。结论:该提取工艺合理,有效成分提取率较高。

Objective： To optimize the extraction technology of total alkaloid in motherwort. Method： The effect of three key factors for the yield of total alkaloid in motherwort such as concentration of alcohol, distilled time and volume of alcohol, were analysed by one-factor-at-a-time-technique method and response surface methodology. Result： The yield model for ex- tracting total alkaloid in motherwort was build, the variance of model is 0.99512, which shows that the model is remarkable, and the best conditions gained： the concentration of alcohol is 96%, the distilled time is 2.7 h, the volume of alcohol is 352 mL. Conclution： The extraction yield of total alkaloid in motherwort is 0.186%.