LPSp对狂犬病疫苗佐剂效应及安全性研究

A study of adjuvant effect and safety of LPSp on rabies vaccine

目的研究成团泛菌脂多糖(LPSp)作为狂犬病疫苗佐剂的可行性。方法将Balb/c小鼠32只随机分为空白对照组(注射生理盐水)、LPSp对照组(注射LPSp)、狂犬病病毒蛋白组(注射狂犬病病毒蛋白)、LPSp实验组(注射LPSp和狂犬病病毒蛋白),分别在第0、7、15、21天经背部皮下免疫接种共4次。在免疫接种后第7、14、21、30、45、60天采集小鼠眼内眦静脉血,分离血清,末次采血后处死小鼠。用酶联免疫吸附试验(ELISA)检测血清抗狂犬病病毒IgG,并进行血液学、血液生化、脏器指数等检查。结果免疫接种4次后,LPSp能明显增强狂犬病病毒蛋白免疫小鼠产生IgG的能力(P<0.05);实验期间各组小鼠未发现明显异常表现,体质量、血液学、血液生化和脏器指数等各组间未见明显差异(P>0.05)。结论 LPSp对狂犬病病毒蛋白具有较好佐剂效应;在本实验条件下,LPSp对小鼠安全性好。

Objective To explore the feasibility of Pantoea agglomerans lipopolysaccharide（LPSp） serving as rabies vaccine adjuvant.Methods 32 Balb/c mice were randomly divided into blank control group（injection of physiological saline）,LPSp control group（injection of LPSp）,rabies virus protein group（injection of rabies virus protein） and LPSp group（injection of LPSp and rabies virus protein）.Totally 4 times subcutaneous vaccination in back were conducted on the 0 th,7 th,15 th and 21th day,respectively.Venous blood samples were collected from eye canthus of mice on the 7 th,14 th,21 th,30 th,45 th and 60 th day after vaccination,serum were separated,and the mice were sacrificed after the last blood collection.Enzyme-linked immunosorbent assay（ELISA） method was employed to detect the serum anti-rabies virus IgG,and hematological test,blood biochemical test as well as viscera index assessment were also conducted.Results After 4 times vaccination,LPSp significantly improved the ability of mice immunized with rabies virus protein to produce IgG（P〈0.05）.No obvious abnormalities were found in mice in each group during experiment.No significantly differences were found among groups in hematological test,blood biochemical test and viscera index assessment（P〈0.05）.Conclusion LPSp exerts a good adjuvant effect on rabies virus protein and shows safety for mice under this experimental condition.