浸润性乳腺癌组织中14-3-3σ、p73α蛋白表达及临床意义

Expression and clinical significance of 14-3-3σ,p73α protein in infiltrating breast cancer

目的探讨表皮细胞特异性标志物14-3-3σ、p73α蛋白在浸润性乳腺癌发生发展中的作用。方法选择我院手术切除的浸润性乳腺癌组织标本94份(浸润癌组),导管原位癌15份(原位癌组),上皮不典型增生15份(不典型增生组),导管内乳头状瘤15份(乳头状瘤组),乳腺增生症20份(增生症组),乳腺癌旁5 cm组织10份(癌旁组),采用免疫组化PV-9000两步法检测各组14-3-3σ、p73α蛋白表达情况,并分析两者间及与浸润性乳腺癌临床病理特征的相关性。结果浸润癌组14-3-3σ蛋白阳性率明显低于其余各组,p73α蛋白阳性率明显高于其余各组,P均<0.05。14-3-3σ蛋白阳性表达与乳腺癌腋窝淋巴结转移、HER-2受体、组织学分级、TNM分期相关,p73α蛋白与腋窝淋巴结转移、ER受体、PR受体、TNM分期相关。14-3-3σ与p73α蛋白表达呈负相关。结论 14-3-3σ和p73α蛋白与浸润性乳腺癌的发生发展相关,两者联合检测可为乳腺癌早期诊断和治疗提供参考。

Objective To investigate the effects of 14-3-3σ and p73α protein expression in the occurrence and development of infiltrating breast cancer.Methods The expression of 14-3-3σ and p73α protein were measured in 94 infiltrating breast cancers tissues（invasive carcinoma group）,15 intraductal carcinoma in situ（carcinoma in situ group）,15 untypical ductal epithelial hyperplasia（untypical hyperplasia group）,15 intraductal papilloma（papilloma group）,20 hyperplastic disease of the breast（hyperplastic group）and 10 peficancerous tissues（peficancerous tissues group） which resected in our hospital by immunohistochemistry PV two-step method,analyzed the correlation of both indexes and which between clinical pathologic features.Results The positive rate of 14-3-3σ protein in invasive carcinoma group was significantly lower than that in the other groups,and the positive rate of p73α protein was significantly higher（all P〈0.05）.The expression of 14-3-3σ protein was significantly correlated with histological grade,TNM stage,axillary lymph node metastasis and HER-2 expression,the expression of p73α protein was significantly correlated with axillary lymph node metastasis,histological grade,TNM stage,ER and PR expression.And there were negative correlations between the expression of 14-3-3σ and p73α protein.Conclusion The protein of 14-3-3σ and p73α may be related to the development of infiltrating breast cancer,and combined detection of which may provide reference for early diagnosis and treatment of breast cancer.