擎天树组培外植体消毒与褐化抑制的研究

Research on the Disinfection and Browning Prevention of Shorea wantianshuea Explants

以擎天树种子和茎段为试验材料,探讨外植体脱毒及防褐化的方法,为建立擎天树微繁无菌体系奠定基础。结果表明:(1)直接利用种胚作为外植体培养脱毒效果较保留种皮好,且消毒过程添加600万单位青霉素处理,能有效降低种子培养污染率;(2)茎段外植体脱毒较困难,青霉素可在一定程度上减低污染率,但消毒效果并不理想,且培养中出现严重褐化现象;(3)添加VC、PVP和活性炭可有效控制外植体褐化现象,在1/2MS培养基中添加0.2mg/LVC、2g/LPVP和0.5g/LAC的效果最好。

The seeds and stem segments of Shorea wantianshuea were used as explants to study the methods of disinfection and browning prevention,which would be helpful to get sterile explants for tissue culture.The results showed that it was better to use embryos explants than the seeds and adding in penicillin with 6 millions units was efficient to lower down the contamination rate during disinfecting.It was difficult for stem segments to be disinfected.Penicillin could reduce contamination rate to some extend,but the effects were not good and there was seriously browning during disinfection.The vitamin C（VC）,polyvinylpyrrolidone（PVP） and activated carbon（AC）could reduce the browning effectively with and optimal combination 0.2 mg/L VC,2 g/L polyvinylpyrrolidone（PVP）and 0.5 g/L activated carbon（AC）in 1/2 MS medium with and optimal combination.