滤光膜对黄檗茎段愈伤组织诱导及3种生物碱含量的影响

Effects of Color Plastic Films on Induction of Callus of Stems and Three Main Alkaloids Contents in Phellodendron amurense

以黄檗茎段为外植体,研究不同颜色滤光膜对其愈伤组织诱导及生物碱(小檗碱、掌叶防己碱、药根碱)含量的影响。将接种到MS+1.5mg/LBA+0.5mg/LNAA+20g/L葡萄糖培养基上的茎段置于不同条件下培养,20天后对其进行形态观察,并取样用于生物量测量及生物碱含量测定。结果表明,各种处理条件下外植体均100%的诱导了愈伤组织,但不同处理对外植体状态、愈伤组织生长和次生代谢产物含量均有不同程度影响。其中,对照、蓝膜处理下诱导的愈伤组织较疏松、颜色鲜艳、生长旺盛,而红膜、绿膜和黄膜则诱导出较少愈伤组织,且呈老化状态。进一步研究表明,对照和蓝膜处理下3种生物碱含量明显高于其他处理。实验表明,对照和蓝膜对愈伤组织生长和次生代谢产物含量的积累效果最好,黄膜次之,绿膜和红膜最差。

The stems of Phellodendron amurense were used as explants to describe the effects of different color plastic films on induction of callus and on the contents of berberine,jatrorrhizine,palmatine.The stems which were inoculated on MS medium containing 1.5 mg/L 6-BA,0.5 mg/L NAA and 20 g/L glucose were treated under different color plastic films.After 20 days of treatment,morphological characteristics of callus were observed and biomass and alkaloid contents were determined.It was shown that all the four treatments induced callus of stems and the induction rate was 100%,but the effects on the status and growth of callus and content of secondary metabolic product were different under various color plastic films.The produced callus tissues under control and blue film were vivid,loose in texture,fast in growth while fewer and aged callus tissues were induced under other films.Further studies showed that the contents of the three alkaloids under control and blue film were higher than others.The results showed that control and blue treatment were the best for growth of tissue and content of secondary metabolic product,followed by yellow and the worst were under green and red ones.