基于纳米金非标记化学发光分析测定K^+

Label-free chemiluminescence detection of K^+ based on gold nanoparticles and aptamer

将核酸识体分子识别模式与纳米金催化发光特性相结合,建立了一种简单、灵敏测定K+的新方法。该方法测定K+的线性范围为7.4×10-8～7.4×10-5mol/L,其检出限为2.9×10-8mol/L。并对浓度为5.6×10-7mol/L的K+重复测定6次,相对标准偏差为3.6%。同时,考察了K+的适配体对K+结合的特异性,结果表明,该方法对于K+的测定具有很好的选择性。

Aptamer＇s molecular recognition and gold nanoparticles＇ catalytic capability for chemiluminescenec（CL） reaction were combined to establish a novel, simple and sensitive CL detection of K ＋. Under the optimum conditions, the linear range for the determination of K ＋ was 7.4× 10 -8 - 7.4 × 10-5 mol/L, and the detection limit（3σ） was 2.9 × 10-8 mol/L. The relative standard deviation of 3.6% （ R. S. D. , n = 6） was obtained. Furthermore, the research results indicate that the determination of K ＋ has good selectivity.