摘要
在 2 0℃条件下 ,采用相对饱和度为 50 %的 (NH4) 2 SO4一步盐析并经 4 5~ 50℃烘干 1 0h得到木聚糖酶粗酶粉 .初步研究了该酶的一些基本性质 ,结果表明 ,该酶在 55℃下 ,pH值为 4 .6~1 0 .5之间较稳定 .最适作用温度与 pH值分别为 50℃和 7.6.该粗酶产品适合造纸工业的纸浆处理 .
The crude xylanase was obtained by salting out in 50% (NH 4) 2SO 4 and dried at 45~50 ℃ for 10 h. Some basic characteristics of this crude enzyme were studied. It was stable at less than 55 ℃ and the range of pH 4.6~10.5.The optimal temperature and pH were 50 ℃ and 7.6 respectively. The results showed that this enzyme was suitable for pulp process in paper industry.
基金
江苏省科委九五攻关项目! (BE960 4 2 )