摘要
对中国各地收集到的数十个红曲米样品中的桔霉素进行了定性及定量测定 ,测定结果表明 ,除少数几个红曲米样品中未测出桔霉素外 ,其它红曲米样品都测出桔霉素 ,红曲红产品中也发现含有桔霉素 .用酵母膏蔗糖培养基 (能检测霉菌是否产生真菌毒素 )培养了近 4 0株红曲霉 ,从菌落形态特征看 ,大多数是Moanascusanka ,定性检测结果表明 ,大多数菌株的培养液中都检测出桔霉素 ,说明这些红曲菌种本身具有产生桔霉素的能力 .初步总结了定性和定量测定高色价红曲米中桔霉素的方法 .
Citrinin contents of red rice samples which were collected from various manufacturers and markets had been detected.The determination results obtained by employing TLC and HPLC methods indicated that most of the red rice samples detected contain citrinin ranged 0.2～140 ug/g.Some products of Monascus Red (the extracts of Monascus products)also contain citrinin. Some isolated Monascus strains (most are Monascus anka according to their colony appearance )were cultivated in YES medium. The supernatants of these Monascus culture were found to contain citrinin by TCL method,indicating the Monascus strains tested are potential citrinin producers.In this paper,we also summarize the methods of TLC and HPLC determination of citrinin for high color value red rice samples.
