尖锐湿疣患者人乳头瘤病毒检测的临床意义

Clinical significance of detecting human papillary virus in patient with condyloma acuminatum

目的探讨尖锐湿疣(CA)患者人乳头瘤病毒(HPV)定量测定与HPV分型的关系。方法用荧光定量聚合酶链反应(FQ-PCR)对133例CA患者疣体组织进行HPV分型及HPV DNA定量检测。结果 133例CA患者经二氧化碳激光治疗后治愈93例,复发40例。133例标本中检出HPV DNA阳性131例(98.50%),110例(82.71%)基因型为HPV6/11,12例(9.02%)为HPV16/18,9例(6.77%)为HPV6/11合并HPV16/18。133例标本中检出HPV DNA阳性131例,阳性率为98.50%。HPV16/18和HPV6/11合并HPV16/18型阳性率均低于HPV6/11型,差异均有统计学意义(P<0.01)。HPV病毒载量不同患者的复发率并无明显差异(P>0.05)。HPV16/18和HPV6/11合并HPV16/18型复发率均高于HPV6/11型(P<0.05或P<0.01)。结论 CA检测HPVDNA能区分高危型HPV和低危型HPV感染,可对CA复发及癌变进行预测。

Objective To discuss the relationship with quantitative determination of condyloma acuminatum（CA） in patient with human papillomavirus（HPV） and HPV type. Methods Used fluorescence quantitative polymerase chain reaction （FQ-PCR） ,detected HPV type and HPV DNA quantification of 133 cases patients with CA. Results 133 cases patients with CA after carbon dioxide laser treatment,93 cases were cured ,40 cases recurrenced. 133 specimens positive for HPV DNA was 131 cases（98.50% ）, 110 cases（82.71% ） genotype was HPV6/11, 12 cases （9. 02% ） genotype was HPV16/18,9 cases （6.77%） was HPV6/11 merger HPV16/18. 133 cases of HPV DNA-positive samples detected in 131 patients,positive rate was 98.50%. HPV16/18 and HPV16/18 HPV6/11 merger-positive rates were lower than those of HPV6/11 type,the difference were statistically significant（ P 〈 0.01 ）. The relapse rate of HPV viral load in patients was no significant difference（ P 〉 0. 05 ）. The relapse rate of HPV16/18 and HPV16/18 type HPV6/11 combined were higher than that of HPV6/11 type （P 〈 0.05 or P 〈 0.01 ）. Conclusion CA can distinguish between HPV DNA detection of high-risk HPV and low-risk HPV infection, and CA to cancer and recurrence can predict.