纤维素分解菌与酵母菌混菌发酵体系对土壤中纤维素物质的降解研究

Degradation of Cellulose in Soil by Mixed Fermentation System with Cellulose-decomposing Bacteria and Yeast

在纤维素分解菌的发酵期间引入酵母菌来解除纤维素分解菌分解纤维素时的产物对纤维素酶的反馈抑制。在不同时间接入酵母菌,找出最适接入时间。通过在最适时间接入不同量梯度的酵母菌,找出最适接种量,同时分析纤维素分解菌与酵母菌在反应器中的动态变化,研究了纤维素分解菌与酵母菌组成的二元混菌体对纤维素酶活性的影响。结果表明:两种菌混菌发酵纤维素的活性较单菌发酵大幅度提高,酵母菌的最适接入时间是72 h,最适接入量为6.4×106 cfu/mL;混菌发酵有利于缩短纤维素生产发酵周期。

The yeast was added to the process of fermenting cellulose-decomposing bacteria in order to relieve feedback inhibition of products, which obtained from cellulose decomposing process, on cellulase. Through adding yeast to the fermentation at different time to find out the optimum time; then, through adding different amounts of yeast at the optimum time to find out the optimum inoculation amount, Meanwhile, the dynamic changes of cellulose-decomposing bacteria and yeast in reactor were analyzed, and the influences of mixed cellulose-decomposing bacteria and yeast on activity of cellulase were also studied. The results showed that the activity of cellulose was significantly increased after fermented by mixed fermentation system; the optimum time for adding yeast was at 72 h; the optimum adding amount was 6.4×10^6 cfu/mL; mixed fermentation is beneficial to shorten fermentation period in cellulose production.