抑制性消减杂交技术及其应用研究进展

Suppression Subtractive Hybridization Technique and Its Application

抑制性消减杂交(SSH)技术是结合抑制性PCR和消减杂交技术发展起来的一种高效分离差异表达基因的方法,具有特异性高、假阳性率低、操作简便、速度快、效率高等优点。利用SSH技术进行不同状态下基因的表达变化分析、差异基因的克隆和鉴定对了解基因表达调控有着重要意义。为此,综述了SSH技术的原理及其在发育调控基因、疾病发生机制、药物研发、性状筛选及环境生物修复等方面的应用研究进展。

Suppression subtractive hybridization（SSH） is a technique of high efficiency for isolation differential expressed genes at transcriptional level,which is based on the use of subtractive hybridization in combination with suppression PCR.Because of its characteristics of high specifi-city,low false positive rate,simple operation and high efficiency,SSH is widely used to study differential gene expression in two differential conditions,playing an important role in cloning andidentifying gene expression difference.The principle and use of SSH technique in the aspects of mechanism of development-related gene regulation,molecule mechanism of disease,mechanism of drug action,selection of fine breed and environmental pollutant biodegradation were analyzed in this review.