回肠Na^+/胆汁酸转运体与高脂血症关系的研究

RELATIONSHIP BETWEEN THE EXPRESSION OF ILEAL NA^+/BILE ACID TRANSPORTER(IBAT) AND HYPERLIPIDEMIA

目的:探讨回肠Na+/胆汁酸性转运体(IBAT)基因表达与高脂血症形成的相关性。方法:取Wistar雄性大鼠60只随机分为2组:普通饮食组(对照组)、高脂饮食组(实验组)。喂养13周后分别提取对照组和实验组Wistar大鼠的空肠末端、回肠末端、盲肠、结肠近端组织,应用RT-PCR和免疫SP法分别检测2组模型Wistar大鼠的空肠末端、回肠末端、盲肠、结肠近端组织的IBAT基因表达强度;对所得结果进行统计分析。结果:IBAT基因表达:结肠近端在对照组和实验组均无表达,空肠末端、回肠末端、盲肠表达实验组强于对照组(P<0.05)。结论:实验组的IBAT表达升高,引起体内胆酸池增大和肝内胆固醇含量升高,从而引起血清胆固醇增加,加速高脂血症的形成。

Objective： To explore the relationship between the expression of ileal Na^＋/bile acid transporter （IBAT）and hyperlipidemia. Methods： Sixty male Wistar rats were randomly divided into normal control diet（n=30） and high fat diet groups （n=30）. Distal ileum, distal jejunum, cecal and proximal colon specimens were obtained 13 weeks after feeding. The expression of IBAT was determined by real-time PCR, and streptavidin-perosidase method was adopted for immunohistochemical staining.Results： The expressions of IBAT in distal ileum, distal jejunum and cecal were significantly higher in high fat diet group （P〈0.05）. Conclusion： Our findings suggest that the expression of IBAT may be responsible for hyperlipidemia.