HPLC测定丹黄祛瘀片中苦参碱和氧化苦参碱的含量

Determination of Contents of Matrine and Oxymatrine in Danhuangquyu Tablets by HPLC

目的:建立丹黄祛瘀片中苦参碱和氧化苦参碱的含量测定方法。方法:采用HPLC,色谱柱为Agilent NH2柱(4.6 mm×250 mm,5μm),流动相为乙腈-无水乙醇-3%磷酸(82∶10∶8),检测波长220 nm,柱温30℃,流速1.0 mL.min-1。结果:苦参碱和氧化苦参碱的线性范围分别为0.385～2.31(r=0.999 6)和0.021 8～0.239 8(r=0.999 2)μg;平均加样回收率分别为99.0%(RSD 0.85%,n=6),97.2%(RSD 1.0%,n=6)。结论:该法简便,快速,结果准确,重复性好,可用于控制丹黄祛瘀片的质量。

Objective：To develop a HPLC method for the determination of matrine and oxymatrine in Danhuangquyu tablets.Method：An Agilent NH2 column（4.6 mm×250 mm,5 μm） was used,the mobile phase was acetonitrile-absolute ethyl alcohol-3% phosphoric acid（82∶10∶8）,the detection wavelength was 220 nm,the column temperature was 30 ℃ and the flow rate was 1.0 mL·min^-1.Result：Matrine showed a good linear relationship at the range of was 0.385-2.31 μg（r=0.999 6）,the average recovery was 99.0%,RSD 0.85%（n=6）;Oxymatrine showed a good linear relationship at the range of was 0.021 8-0.239 8 μg（r=0.999 2）,the average recovery was 97.2%,RSD 1.0%（n=6）.Conclusion：The method is accurate and reliable,and it can be used for the quality control of Danhuangquyu tablets.