钩藤饮片HPLC指纹图谱研究

Study on Uncariae Ramulus Cum Uncis by HPLC-Fingerprints

目的:建立钩藤饮片的HPLC指纹图谱评价体系,并结合聚类分析判别方法,提高钩藤饮片质量控制标准。方法:流动相乙腈-0.385%乙酸铵-冰乙酸水溶液梯度洗脱,检测波长245 nm。对27批不同商品地钩藤饮片进行测定,以中药色谱指纹图谱相似度评价软件建立了钩藤饮片标准指纹谱,并计算饮片的相似度。结果:方法精密度、重复性与稳定性良好。将钩藤饮片聚类分为五类,确定17个共有峰,80%的样品相似度>0.87。结论:建立的HPLC指纹图谱和聚类分析方法有助于钩藤饮片质量全面评价体系的建立。

Objective：To establish the method of HPLC fingerprint and hierarchical cluster analysis for quality control of Uncariae Ramulus Cum Uncis.Method：About 27 samples from different sources were analyzed by HPLC.Acetonitrile and 0.385% ammonium acetate-acetic acid buffer were used as mobile phase by gradient eluting.Detection wavelength was set at 245 nm and column at 35 ℃.By the similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine（Version 2004A）,mean chromatogram was generated as the representative standard fingerprint.Result：Seventeen main marker peaks were selected in the standard fingerprint,and the similarity of 80% samples was over 0.87.The method is high sensitivity and good reproducibility and the Uncariae Ramulus Cum Uncis could be divided into five grades through the results of hierarchical cluster analysis.Conclusions：The fingerprint study and hierarchical cluster analysis method can be used for the quality control of Uncariae Ramulus Cum Uncis.