博落回提取物对实验性肝纤维化的防治作用

Experimental Study on Antagonizing Liver Fibrosis of Macleaya cordata Extract

目的:观察博落回提取物(Macleaya cordata extract,MCE)对实验性肝纤维化的防治作用。方法:采用CCl4复合因素诱导的大鼠肝纤维化和小鼠血吸虫肝纤维化2种模型,观测MCE干预后肝指数、肝功能生化指标[丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)]、血清纤维化指标[Ⅲ型前胶原(PCⅢ)、层黏连蛋白(LN)、透明质酸(HA))、脂质过氧化指标[谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)、超氧化物歧化酶(SOD)),肝组织Ⅰ型胶原(CoⅠ)、Ⅲ型胶原(CoⅢ)及羟脯氨酸(Hyp)表达的变化,并做肝脏病理学检查。结果:在CCl4肝纤维化大鼠模型中,MCE各剂量预防组肝指数,AST含量及CoⅠ表达明显降低,高剂量预防组LN及CoⅢ表达明显降低;中剂量预防组MDA含量及CoⅢ表达明显降低;MCE治疗组肝指数、HA,MDA含量及CoⅢ表达明显降低;经MCE防治后大鼠肝纤维化程度有不同程度减轻。在小鼠血吸虫肝纤维化模型中,MCE预防组小鼠Hyp及CoⅠ表达明显降低;高剂量治疗组小鼠肝指数、PCⅢ,LN,HA,AST,ALT含量及CoⅠ表达显著降低,中剂量治疗组PCⅢ,HA,AST,ALT含量明显降低;MCE治疗给药能明显改善模型小鼠肝脏病变。结论:MCE对实验性肝纤维化有一定的防治作用,其机制可能与其保护肝细胞膜、减轻肝脏炎症及抗脂质过氧化作用有关。

Objective：To investigate the preventive and therapeutic effects of Macleaya cordata extract（MCE） on experimental liver fibrosis.Method：Experimental liver fibrosis models were induced by injection of carbon tetrachloride in combination with the drinking of 5% alcohol on rats and Schistosoma japomicum infection in mice.The effect of MCE on anti-hepatic fibrosis was evaluated by comparing the liver index,tissue biochemical indices（ALT,AST）,lipid peroxidation indices（GSH-Px,MDA,SOD）,serum fibrosis indices（HA,PCⅢ,LN）,the expression levels of Hyp and collagen type Ⅰ,Ⅲ（CoⅠ,CoⅢ）,the liver pathology before and after MCE intervention.Result：In CCl4-induced liver fibrosis model in rats,Compared with model group,the liver index,the level of AST,the expression of CoⅠwere obviously decreased in MCE preventive groups,and high-dose preventive MCE could significantly reduce the content of LN and the expressionof CoⅢ,middle-dose preventive MCE could significantly reduce the content of MDA and the expression of CoⅢ.The liver index,the contents of HA,MDA,the expression of CoⅢ were significantly decreased in MCE therapeutic group;in S.japomicum-induced liver fibrosis model in mice,preventive MCE could significantly reduce the content of ALT,the expression of Hyp and CoⅠ;the liver index,the contents of PCⅢ,LN,HA,AST,ALT in serum and the expression of CoⅠwere significantly decreased in high-dose MCE therapeutic group,and middle-dose therapeutic MCE could significantly reduce the levels of PCⅢ,HA,AST,ALT.In addition,the hepatic histopathology were also improved in varying degrees after MCE intervention.Conclusion：MCE has preventive and therapeutic effects on experimental liver fibrosis by inhibiting hepatic lipid peroxidation and alleviating the inflammation.