葛根素对大鼠体外循环后心肌缺血再灌注损伤的保护作用及抗氧化应激机制的探讨

Protection of Puerarin on Oxidative Stress Induced by Acute Myocardial Ischemia-Reperfusion Injury in Rats in Cardiopulmonary Bypass

目的:探讨葛根素对大鼠体外循环后心肌缺血再灌注损伤(myocardial ischemia-reperfusion injury,MIRI)的保护作用及抗氧化应激机制。方法:取健康雄性SD大鼠75只,随机分为5组:即假手术组(给予等体积的生理盐水)、MIRI模型组(给予等体积的生理盐水)、葛根素低、中、高剂量组(2,5,10 mg.kg-1 3个剂量)。于再灌注开始时在储血槽内加入稀释葛根素10mL。在全麻手术下制造大鼠体外循环模型后,随即阻断大鼠升主动脉造成心肌缺血30 min然后开放升主动脉后再灌注180 min造成大鼠心肌缺血再灌注损伤模型(灌注24 h,用于测定心肌梗死面积)。实验组和对照组分别给予葛根素和生理盐水。实验完成后留取大鼠心脏标本,观察大鼠心肌缺血区的心肌细胞凋亡情况;收集血清测定其抗氧化应激的指标:超氧化物歧化酶(SOD),丙二醛(MDA),谷胱甘肽(GSH),谷胱甘肽过氧化物酶(GSH-Px)。结果:与模型组相比,葛根素的应用减少了MIRI大鼠的心肌细胞凋亡、心梗面积和血清中丙二醛的含量,增加了血清中超氧化物歧化酶、谷胱甘肽过氧化物酶的活性和谷胱甘肽的含量,并且随着剂量的增加保护效果尤为明显。结论:葛根素对MIRI大鼠具有抗氧化应激的作用,它能够剂量依赖性的减少心肌细胞凋亡,最终减少心肌梗死面积。

Objective：To investigate the protective mechanism of puerarin on acute myocardial ischemia-reperfusion injury（MIRI）in rats,and to explain the antioxidative mechanism involved.Method：Rat MIRI model was induced by ischemia for 30 min and reperfusion for 180 min.At the end of the 3 h reperfusion period（or 24 h for infarct size）,myocardial infarct size,myocardial apoptosis and the activity of antioxidative enzymes were measured.Result：Puerarin reduced infarct size,myocardial apoptosisand the serum level of malondialdehyde,increased the activity of superoxide dismutase and glutathione-peroxidase activities and serum level of glutathione.Conclusion：Puerarin exerts significant cardioprotective effects against MIRI in rats,likely through its antioxidation and antilipid peroxidative properties.