两肾一夹型高血压大鼠模型的改良及评价

Evaluation and Modification of Goldblatt 2K1C Renovascular Hypertensive Rat Models

目的:采用Goldblatt经典肾性高血压大鼠模型并加以改进,建立简易稳定的两肾一夹型高血压大鼠模型。方法:将70只雄性SD大鼠,随机分为模型组和假手术组,其中模型组大鼠从背部开口以丝线结扎左肾动脉,假手术组大鼠除不结扎左肾动脉,余步骤同模型组。术后测定4周内不同时间点血压及观察一般情况对所建模型进行评价。结果:模型组术后成活率达94.5%,成模率达89.1%模型组大鼠自造模后3 d血压开始升高,此后呈持续性升高,与实验前和假手术组比较均保持显著性差异(P<0.05)。结论:从背部开口以丝线结扎大鼠左肾动脉是一种安全性高和手术操作简便的新方法。

Objective：To establish two-kidney one-clip（2K1C） renovascular hypertensive rat model with improved Goldblatt classic hypertensive rat model.Method：Seventy male SD rats were randomly divided into two groups：modol group and sham-operation group.Left renal artery of model rats was occluded by clips from back incising.Left renal artery of control rats was liberated but not narrowed,other operation was as same as the model rats.After the operation,we evaluated results by observing blood pressure.Result：The model rats had high survival rate（94.5%） after operation.The model establishing rate was 89.1%.Compared with control rats and the results before the surgery,the blood pressure in model rats was significantly increased after 3 days of surgery and increased continuously（P〈0.05）.Conclusion：The modification of 2K1C renovascular hypertensive models by narrowing left renal arteries by medical silk suture from back incising in rats is a simple operating and safe approach,suggesting the practical utility of this new method.