5种中药醇提物体外抑制2种革兰阳性菌的活性

Effect in vitro Inhibition of Five Ethanol-extractions from Traditional Chinese Medicine on Two Kinds of Gram Positive Bacteria

目的:研究丹参、丁香、厚朴、金银花、大黄这5种中药醇提物对痤疮丙酸杆菌和金黄色葡萄球菌的抑菌活力,进而通过正交试验考察不同配比对这2种革兰阳性菌的抑菌作用。方法:丹参、丁香、厚朴、金银花、大黄醇提物浓缩至0.2 g.mL-1,通过测量抑菌圈直径考察不同中药醇提物与醇提物配伍后对这2种细菌的抑菌效果。结果:痤疮丙酸杆菌对丹参醇提物为高度敏感,抑菌圈直径为(23.27±3.68)mm,对丁香、厚朴醇提物为中度敏感,为(18.82±2.27),(17.78±1.88)mm;金黄色葡萄球菌对丁香和厚朴醇提物为高度敏感,抑菌圈直径分别为(21.94±0.74),(19.52±1.09)mm,对大黄和丹参为中度敏感,抑菌圈直径为(14.43±2.24),(16.01±0.88)mm。丹参、厚朴、丁香醇提物最佳复配比例为1∶1∶1,其对痤疮丙酸杆菌和金黄色葡萄球菌的抑菌圈直径分别为(20.07±1.26),(20.57±0.38)mm。结论:丹参、厚朴、丁香的醇提物对这2种菌的抑菌活力均为高度敏感,抑菌作用最佳。丹参、厚朴、丁香的醇提物对2种菌抑菌活性的最佳配比为1∶1∶1。

Objective：To study effect of ethanol-extractions of Salvia miltiorrhiza,Syzygium aromaticum,Magnolia officinalis,Louicera japonica,Rheum palmatum this 5 kinds of traditional Chinese medicine on bacteriostasis activity to Propionibacterium acnes and Staphylococcus aureus,and then investigate different proportion of the ethanol-extractions by orthogonal test on antimicrobial effect of both Gram positive bacteria.Method：The ethanol-extractions of S.miltiorrhiza,S.aromaticum,M.officinalis,L.japonica,R.palmatum were enriched to 0.2 g·mL-1.Inhibition zone diameters were measured to evaluate the bacteriostatic effects and the different ethanol-extraction compatibility.Result：Propionibacterium acne was highly sensitive to S.miltiorrhiza ethanol-extraction with the inhibition zone diameter of（23.27±3.68） mm.It showed moderate sensitivity to Syzygium aromaticum and Magnolia officinalis ethanol-extractions,the inhibition zone diameters were（18.82±2.27） and（17.78±1.88） mm accordingly.Staphylococcus aureus was highly sensitive to S.aromaticum and M.officinalis ethanol-extractions,the inhibition zone diameters were（21.94±0.74） and（19.52±1.09） mm respectively.It showed moderate sensitivity to R.palmatum and S.miltiorrhiza ethanol-extractions,the inhibition zone diameters were（14.43±2.24） and（16.01±0.88） mm.The best mixed proportion of alcohol extract of S.miltiorrhiza,M.officinalis and S.aromaticum was 1∶1∶1.The inhibition zone diameters of the compound to Propionibacterium acne and Staphylococcus aureus were（20.07±1.26） and（20.57±0.38） mm respectively.Conclusion：Alcohol extracts of S.miltiorrhiza,M.officinalis,S.aromaticum have high antibacterial activity to two species of bacteria.The best mixed propotion for alcohol extracts of S.miltiorrhiza,M.officinalis,S.aromaticum on antibacterial activity to two bacteria appears tobe 1∶1∶1.