莪术醇对人胚肺成纤维细胞增殖的影响

Inhibiting Proliferation of Human Embro Fibroblasts by Curcumol

目的:探讨莪术醇对人胚肺成纤维细胞增殖抑制作用。方法:MTT法观察莪术醇对人胚肺成纤维细胞增殖的抑制作用;天狼猩红染色法测定细胞胶原的分泌能力,流式细胞术检测细胞周期时相分布。结果:50～200 mg.L-1莪术醇作用细胞后能抑制人胚肺成纤维细胞的增殖和制胶原沉积,以96 h效果最为明显,并阻滞细胞停滞于G0/G1期,降低S期和G2/M期细胞时相比例。结论:莪术醇通过将细胞周期阻滞于G0/G1,减少DNA复制,抑制人胚肺成纤维细胞增殖和细胞分泌胶原。

Objective：To explore the inhibiting the proliferation and the mechanism of human embro fibroblasts（MRC-5） by curcumol.Method：The effects of curcumol on the proliferation of MRC-5 were determined by MTT assay.The cotents of collagen accumulated were determined by the optical density at 540 nm of picro-sirius red staining.The cell cyclic retardation was examined by using flowcytometry.Result：MTT showed that curcumol（50-200 mg·L^-1） had an inhibitory effect on the proliferation and had reduced collagen of secretion in MRC-5.The better time in the 96 hour.The cell cycle analysia showed that curcumol（50-200 mg·L^-1） increased the percentage of G0/G1 phase cells,updown the percentage of S and G2/m phase cells.Conclusion：Curcumol can increase the percentage of G0/G1 phase cells,delay cell replication,inhibit the proliferation and collagen of secretion of MRC-5.