摘要
目的研究比较银杏内酯B与姜黄素联合三氧化二砷(As2O3)治疗白血病过程中对心肌细胞氧化损伤的保护作用。方法对人白血病细胞株NB4进行培养,设立对照组、As2O3组、银杏内酯B组及姜黄素组。对照组:原营养液继续培养;As2O3组:加入As2O32μmol/L;银杏内酯B组:加入As2O32μmol/L+银杏内酯B 0.1mmol/L;姜黄素组:加入As2O30.5μmol/L+姜黄素50μmol/L。以分光光度法检测超氧化物岐化酶(SOD)、谷胱甘肽过氧化酶(GSH-PX),流式细胞仪检测细胞内活性氧(ROS)水平。结果经姜黄素、银杏内酯B干预后的NB4细胞超微结构都能得到明显改善,姜黄素、银杏内酯B均显著提高了NB4细胞内ROS、SOD含量及GSH-PX活力,姜黄素组较银杏内酯B组显著提高NB4细胞内ROS、SOD含量及GSH-PX活力(P<0.01)。结论姜黄素、银杏内酯B均可联合As2O3,上调机体ROS、SOD水平,提高GSH-PX的活性,拮抗自身氧化损伤,姜黄素对心肌细胞氧化损伤的保护作用优于银杏内酯B,为进一步提高白血病临床疗效打开了新思路。
Objective To compare the effect to oxidative damage of myocardial cell by the combination of ginkgolide B with As2O3 and the combination of curcumin with As2O3 for the treatment of leukemia. Methods The NB4 cells were cultured and divided into control group,As2O3 group,ginkgolide B group and curcumin group.Control group was continuously cultured with the original medium,As2O3 group was added with As2O3 2 μmol/L,ginkgolide B group was added with As2O3 2 μmol/L ＋ ginkgolide B 0.1 mmol/L,curcumin group was added with As2O3 0.5 μmol/L＋curcumin 50 μmol/L.The amounts of superoxide dismutase（SOD） and glutathione peroxidase（GSH-PX） were tested by spectrophotometry.The expression of reactive oxygen species（ROS） was tested by flow cytometry. Results The intervention by curcumin or ginkgolide B improved significantly the ultrastructure of NB cells.Curcumin or ginkgolide B either raised significantly ROS,SOD contents and GSH-PX activity,and the efficacy was higher by the combination of curcumin with As2O3 than by the combination of ginkgolide B with As2O3. Conclusion The combination of curcumin with As2O3 can antagonize oxidative damage of organism and provide a new idea of clinical efficacy of leukemia.
出处
《临床荟萃》
CAS
2012年第2期125-126,131,共3页
Clinical Focus
