FITC柱前衍生生物胺HPLC-LIFD的分离

FITC pre-column derivatization of biogenic amines in the separation of the HPLC-LIFD

采用异硫氰酸荧光素（FITC）作为柱前衍生试剂，对酱油中的生物胺衍生化，采用高效液相色谱（HPLC）与激光诱导荧光检测器（LIFD）联用技术，对衍生产物进行了检测。建立了酱油中生物胺的衍生及分离方法，考察了衍生温度、缓冲液pH、衍生反应时间等因素对生物胺衍生化的影响，考察了流动相、流速、温度等条件对分离效果的影响。衍生条件为：衍生温度50℃，缓冲液pH8～10，衍生时间12h；分离条件为：流动相：乙腈一水，流速：1．0mL／min，室温。

In this paper, fluorescein isothiocyanate （FITC） as a pre-column derivatization reagent, the derivatives of biogenic amines in soy-based, high-performance liquid chromatography （HPLC） with laser-induced fluorescence detector （LIFD） in conjunction with technology, the product of the derivative were tested. Established derivatives of biogenic amines in soy sauce and separation, this study investigated the derivative temperature, buffer pH, reaction time and other factors derived fi＇om biological amine derivative of the impact study of the mobile phase, flow rate,temperature and other conditions on the separation effect. Derivative conditions： derived temperature 50℃, buffer pH 8-10, derivative time 12 h, separation conditions： mobile phase： acetonitrile - water, flow rate： 1.0mL/min, at room temperature.