甘肃省汉族、回族人非综合征唇腭裂与转化生长因子-α基因多态性的相关性对比

Association between transforming growth factorαgene variant and nonsyndromic cleft lip with or without cleft palate of Han/Muslin people in Gansu province

目的研究甘肃省汉族和回族人非综合征唇腭裂与转化生长因子-α基因多态性的相关性。方法采用聚合酶链反应-限制性酶切片段长度多态性方法 ,对50例汉族非综合征唇腭裂患者与50例正常汉族人,50例回族非综合征唇腭裂患者与50例回族正常人进行TGF-α基因检测分析。结果汉族、回族人非综合征唇腭裂患者的等位基因频率较正常对照组明显增高,差异有统计学意义(P<0.05);汉族和回族人非综合征唇腭裂患者等位基因频率、基因型均差异无统计学意义。结论 TGF-α基因多态性与甘肃省汉族、回族人群非综合症唇腭裂的发生可能有关联,但在汉族和回族患者之间无特异性选择。

Objective To study the association between transforming growth factor a gene （TGF- a） variant and nonsyndromic cleft lip with or without cleft palate （NSCL/P） of Han and Muslin people in Gansu province. Methods TGF-a variant was detected by using RFLP-PCR for DNA samples of the 50 with NSCL/P in Han people and 50 normal people, 50 with NSCL/P in Muslin people and 50 normal people. Results It was found that allele frequency at TGF-a/TaqI locus in NSCL/P groups was significantly higher （P 〈0.05）. Conclusion The TGF-a gene polymorphism is related to the occurrence of NSCL/P in Han and Muslin people in Gansu province, but has no particularity between Han and Muslin people with NSCL/P.