CXCR4在胃癌中的表达及其意义

Correlation of CXCR4 expression with metastasis tissues in gastric carcinoma

目的:研究趋化因子受体CXCR4在胃癌中的表达,探讨其与淋巴结、肝转移及预后的关系。方法:免疫组化法检测胃癌原发灶、正常胃黏膜组织、淋巴结转移癌组织以及肝转移组织CXCR4表达情况。RT-PCR检测其CXCR4mRNA在4株胃癌细胞株中的表达。结果:正常胃黏膜组织、胃癌组织、淋巴结转移癌组织以及肝转移组织中CXCR4阳性率分别为15.5%、51.5%、72.2%、73.5%;转移组织阳性表达率明显高于原发肿瘤。CXCR4mRNA高表达于2株人胃癌细胞株。胃癌组织CXCR4阳性表达率与TNM临床分期、淋巴结转移、肝转移密切相关(P<0.05),而与患者年龄、性别、肿瘤所在部位及病理分化程度无关。CXCR4阳性表达组3年生存率明显低于阴性表达组(P<0.05)。结论:CXCR4阳性表达与胃癌的淋巴结、肝转移有关,有助于预后判断。

Objective：To investigate the expression of chemokine receptor CXCR4 in gastric carcinoma and its correlation with lymphatic and hepatic metastasis and prognosis. Methods：The expression of chemokine re- ceptor CXCR4 protein was detected by immunohistochemistry in 20 normal gastric mucosa tissue, 64 gastric carcinoma tissuse, 34 lymphatic metastasis and 18 hepatic metastasis. Simultaneously, the expression of chemokine receptor CXCR4 mRNA in 4 gastric carcinoma cell lines was examined by reverse transcription poly- merase chain reaction. Results：The positive rate of CXCR4 protein expression was 15.5 % in normal gastric mucosa tissues, 51. 5% in gastric carcinoma, 72. 2% in lymphatic metastasis and 73. 5% in hepatic metastasis. Two of 4 gastric carcinoma cell lines expressed CXCR4 mRNA. The positive rate of CXCR4 protein expression was correlated with lymph node metastasis, clinical staging and liver metastasis （P 〈 0.05）, but not with the sex and age of patients,tumor location, T staging and pathological types. 3--year survival rate in positive expression group was obviously lower than negative expression group. Conclusion： Positive expression of CXCR4 by gastric cancer cells is significantly associated with lymphatic and hepatic metastasis in gastric cancer, which can be used as potential predictive factors for the prognosis of gastric cancer.