电刺激小脑顶核对缺氧缺血新生大鼠睫状神经营养因子蛋白的影响

Effect of Fastigial Nucleus Stimulation on Ciliary Neurotrophic Factor Protein in Newborn Rats with Hypoxic-ischemic Brain Damage

目的探讨电刺激小脑顶核对缺氧缺血脑损伤(HIBD)新生大鼠睫状神经营养因子(CNTF)蛋白及学习记忆功能的影响。方法将大鼠随机分为假手术组、模型组和电刺激组,每组又分为7 d和14 d 2个亚组,每个亚组6只。采用结扎左侧颈总动脉并吸入氮氧混合气体2 h制作新生大鼠HIBD动物模型,电刺激组于造模后第2天开始电刺激治疗,强度25 mA,频率5 Hz,20min/次,每天2次。分别于干预后7 d、14 d用Y-型迷宫检测。HE染色法光镜下观察脑神经细胞和神经纤维的病理变化,免疫组化检测脑组织CNTF蛋白水平的表达。结果电刺激组总反应时间明显短于模型组(P<0.01),主动回避率及正确反应率均明显高于模型组(P<0.01),皮质及海马周围CNTF蛋白面积与积分光密度值较模型组增多(P<0.05)。结论电刺激小脑顶核可以提高脑组织CNTF蛋白水平表达,促进脑损伤大鼠学习记忆能力的恢复。

Objective To explore the effect of fastigial nucleus stimulation on ciliary neurotrophic factor（CNTF） protein and learning memory ability in newborn rats with hypoxic-ischemic brain damage（HIBD）.Methods 36 newborn rats were randomly divided into sham operation group,model group and electrical stimulation group.The model group and the electrical stimulation group were prepared by ligation of left common carotid artery with a temporary systemic hypoxia for 2 h.The stimulation group received electric stimulation treatment from the second day after modeling,25 mA,5 Hz,20 min a time,2 times a day.Y-type maze was used to detect the learning and memory function 7 d and 14 d after treatment respectively.Hematoxylin-Eosin staining was used to observe the pathological change of brain nerve cells and nerve fibres.Immunohistochemistry was used to detect the expression of CNTF.Results The total reaction time of the electrical stimulation group was less than the model group（P0.01）,the active avoidance rate and correctly reflect rate were higher than model group（P0.01）,the expression of CNTF was higher than the model group（P0.05）.Conclusion Fastigial nucleus stimulation can increase the expression level of CNTF,facilitate the recovery of the learning memory ability of rats with brain damage.