摘要
目的探讨组织芯片技术在脑胶质瘤研究中的应用价值。方法构建包括50例人脑胶质瘤标本的组织芯片,运用免疫组织化学法检测细胞增殖核抗原Ki-67的表达,并用免疫组织化学法和原位杂交法检测突变型P53蛋白及野生型p53基因的表达。结果 Ki-67表达水平与肿瘤病理分级之间有关(P<0.05)。突变型P53蛋白与野生型p53 mRNA之间呈显著性负相关(P<0.001)。突变型P53蛋白与Ki-67的表达水平之间存在显著性正相关(P<0.05)。结论应用组织芯片技术大规模高效检测胶质瘤临床组织样本是可行的,具有重要的实用价值和广阔的应用前景。
Objective To investigate the application of tissue microarray（TMA） technique in human brain gliomas.Methods The TMAs containing 50 glioma specimens of all pathological grades were constructed.The immunohistochemistry and in situ hybridization techniques were used to detect the expressions of Ki-67,mutant P53 protein and wild-type p53 mRNA.Results The expression of Ki-67 was significantly associated with the pathological grades（P[]0.05）.There was significant correlation between the expression of mutant P53 protein and wild-type p53 mRNA（P[]0.001）,as well as p53 mutation and Ki-67（P[]0.05）.Conclusion It＇s feasible and valuable to utilize TMA technique in research on human brain gliomas.
出处
《中国康复理论与实践》
CSCD
2011年第12期1124-1127,共4页
Chinese Journal of Rehabilitation Theory and Practice
基金
北京市自然科学基金项目(7102028)
关键词
胶质瘤
组织芯片
免疫组织化学
原位杂交
glioma
tissue microarray
immunohistochemistry
in situ hybridization
