稳定期慢性阻塞性肺疾病患者诱导痰及血清中细胞因子的表达

The express of interleukin-1β(IL-1β),interlenkin8(IL-8) and tumor necrosis factor-α(TNF-α) at different stage of stable chronic obstructive pulmonary disease(COPD)

目的探讨不同严重程度稳定期慢性阻塞性肺疾病(COPD)患者血清及诱导痰液中细胞因子白细胞介素-1β(IL-1β)、白介素-8(IL-8)、肿瘤坏死因子(TNF-α)的表达。方法分别纳入健康吸烟成人90例、稳定期COPD患者100例为研究对象。采用放射免疫法测定诱导痰及血清细胞因子IL-8、IL-1β、TNF-α的水平。结果稳定期COPD患者诱导痰和血清中IL-8、IL-1β、TNF-α较健康吸烟组均升高,差异具有统计学意义。同时稳定期COPD患者血清和诱导痰中三种细胞因子水平与肺功能呈显著负相关。结论 IL-1β、IL-8,TNF-α均参与了COPD的发生和发展过程,其诱导痰和血清水平可能是COPD严重程度的一个炎性标志。

Objective To evaluate the express of Interleukin-1β（IL-1β）,Interlenkin-8（IL-8） and tumor necrosis factor-α（TNF-α） in induced sputum and serum in different stages of stable chronic obstructive pulmonary disease（COPD）.Methods 90 health smoking controls and 100 stable chronic obstructive pulmonary disease（COPD） patients were enrolled.The levels of IL-1β,IL-8 and TNF-α in induced sputum and blood serum were detected by radioimmunoassay.Results The levels of IL-1β,IL-8 and TNF-α in stable chronic obstructive pulmonary disease（COPD） patients were much higher than that in health smoking controls.There were an inverse correlation between FEV1 and level of IL-1β,IL-8 and TNF-α in induced sputum and blood serum.Conclusion IL-1β,IL-8 and TNF-α may play important roles in the mechanism of airway inflammation in stable chronic obstructive pulmonary disease（COPD）.The level of IL-1β,IL-8 and TNF-α in induced sputum and serum may be a suggestion of the order of severity of airway inflammation in stable chronic obstructive pulmonary disease（COPD）.