固相萃取-超高效液相串联质谱同时测定食品中苏丹橙G、二甲基黄、苏丹黑B

Determination of Sudan Orange G,Dimethyl Yellow,Sudan Black B in Food by SPE-UPLC-MS/MS Method

建立了食品中苏丹橙G、二甲基黄、苏丹黑B非法色素的固相萃取超高效液相串联质谱测定方法。以10%乙醇的丙酮溶液作为提取溶剂,利用MAX强阴离子交换固相萃取柱对样品进行净化。检出限苏丹橙G、二甲基黄为1.0μg/kg,苏丹黑B为10μg/kg。线性范围苏丹橙G、二甲基黄为2.5-2500μg/kg,苏丹黑B为25-25000μg/kg,加标回收为78%～105%。本方法灵敏、准确高效,可对非法油溶色素进行测定及确证。

To established a method for simultaneous determination of Sudan orange G,Dimethyl yellow,Sudan black B in food by solid-phase extraction and ultra performance liquid chromatography electrospray ionization-tandem mass spectrometry（SPE-UPLC-MS/MS） method.The samples were extracted by acetone containing 10 % ethanol and cleaned-up by MAX solid phase extraction column.The qualitative detection limits of the method for Sudan Orange G,Dimethyl yellow were 1.0 μg/kg and for Sudan black B was 10 μg/kg.The linear range were 2.5-2500 μg/kg for Sudan orange G,Dimethyl yellow were 1.0 μg/kg and for Sudan black B was 10 μg/kg.The average recoveries of adding standard were 78 %～105 %.The method is sensitive,accurate and could be used for detecting 3 kinds of oil-soluable illegal pigments in food.